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GeneCorner plasmid details

Last data update: 30 November 2020 04:30 CET

Plasmid name: pATMIFNG (LMBP 1597)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p1597.gb (View with Genome Compiler)
p1597.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse interferon γ cDNA (IFNG)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153
Further information: pATMIFNG was derived from pAT153 by cloning the mouse interferon γ cDNA (mIFNG) into the unique PstI site of the ampicillin resistance gene via dC/dG-tailing.
The length and the sequence of the 5' and 3' untranslated regions of the mouse IFNγ insert are not completely known.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727580.1.
The nucleotide sequence of the mouse IFNG cDNA corresponds with the EMBL Nucleotide Sequence Database accession number K00083.1.
Other name of the plasmid is pAT10.
EMBL Accession number: K00083.1, view at EMBL, GenBank, DDBJ
LT727580.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 08/07/2003
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AatII/SmaI, BamHI, NdeI/SalI, PstI, RsaI/StyI and StyI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12
Host reference: -
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pATMIFNG (LMBP 1597) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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