Last data update: 24 January 2024 16:39 CET
Plasmid name: pATMIFNG (LMBP 1597)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1597.gb
(View with Genome Compiler) p1597.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse interferon γ cDNA (IFNG) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pAT153 |
| Further information: | pATMIFNG was derived from pAT153 by cloning the mouse interferon γ cDNA (mIFNG) into the unique PstI site of the ampicillin resistance gene via dC/dG-tailing. The length and the sequence of the 5' and 3' untranslated regions of the mouse IFNγ insert are not completely known. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727580.1. The nucleotide sequence of the mouse IFNG cDNA corresponds with the EMBL Nucleotide Sequence Database accession number K00083.1. Other name of the plasmid is pAT10. |
| EMBL Accession number: | K00083.1, view at EMBL, GenBank, DDBJ LT727580.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 08/07/2003 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AatII/SmaI, BamHI, NdeI/SalI, PstI, RsaI/StyI and StyI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pATMIFNG (LMBP 1597) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.