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GeneCorner plasmid details

Last data update: 01 December 2020 04:11 CET

Plasmid name: pATMTNF1 (LMBP 2527)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2527.gb (View with Genome Compiler)
p2527.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse tumor necrosis factor cDNA (Tnf, TNF)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153
Further information: The plasmid was constructed as follows: dG/dC-tailed mouse tumor necrosis factor cDNA (mTNF) was inserted into the filled-in PstI site of pAT153.
The plasmid differs from pATMTNF3 in the length of the 3' and 5' untranslated regions.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727523.1.
Name mentioned in Fransen et al. (1985) is p-mTNF-1.
Other name of the plasmid is pmTNF1.
EMBL Accession number: M11731, view at EMBL, GenBank, DDBJ
LT727523.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 17/01/1992
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindIII, PstI, StuI/XmnI and XcmI/XmnI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr W. Fiers(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Fransen et al., Nucleic Acids Res. 13 (1985), 4417-4429 [PMID: 2989794]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH1(λ)
Host reference: Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pATMTNF1 (LMBP 2527) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Fransen et al., 1985.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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