Last data update: 24 January 2024 16:39 CET
Plasmid name: pATMTNF1 (LMBP 2527)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2527.gb
(View with Genome Compiler) p2527.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse tumor necrosis factor cDNA (Tnf, TNF) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pAT153 |
| Further information: | The plasmid was constructed as follows: dG/dC-tailed mouse tumor necrosis factor cDNA (mTNF) was inserted into the filled-in PstI site of pAT153. The plasmid differs from pATMTNF3 in the length of the 3' and 5' untranslated regions. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727523.1. Name mentioned in Fransen et al. (1985) is p-mTNF-1. Other name of the plasmid is pmTNF1. |
| EMBL Accession number: | M11731, view at EMBL, GenBank, DDBJ LT727523.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/01/1992 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindIII, PstI, StuI/XmnI and XcmI/XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Fransen et al., Nucleic Acids Res. 13 (1985), 4417-4429 [PMID: 2989794] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH1(λ) |
| Host reference: | Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219 |
| Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pATMTNF1 (LMBP 2527) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Fransen et al., 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.