Last data update: 24 January 2024 16:39 CET
Plasmid name: pATMTNF3 (LMBP 2528)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | patmtnf3.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse tumor necrosis factor cDNA (Tnf, TNF) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pAT153 |
| Further information: | This plasmid was constructed as follows: GC-tailed cDNA from mouse TNF was inserted into the filled-in PstI site of pAT153. The orientation of the inserted fragment is unknown. This plasmid differs from pATMTNF1 in the length of the 3' and 5' untranslated regions. Name mentioned in Fransen et al. (1985) is p-mTNF-3. Other name of the plasmid is pmTNF3. |
| EMBL Accession number: | M11731, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/01/1992 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Fransen et al., Nucleic Acids Res. 13 (1985), 4417-4429 [PMID: 2989794] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH1(λ) |
| Host reference: | Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219 |
| Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pATMTNF3 (LMBP 2528) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Fransen et al., 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.