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GeneCorner plasmid details

Last data update: 03 December 2020 04:26 CET

Plasmid name: pATMx (LMBP 2390)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2390.gb (View with Genome Compiler)
p2390.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Mouse Mx promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153; pSP64Mx
Further information: The plasmid was constructed as follows: the Mx promoter was isolated as a HindIII-EcoRI fragment from pSP64Mx, and ligated between the HindIII and EcoRI sites of the pAT153 vector.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727508.1.
EMBL Accession number: LT727508.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 22/04/1991
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglI, EcoRI and HindII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pATMx (LMBP 2390) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vanhoenacker and Prof. Dr G. Haegeman.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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