Last data update: 24 January 2024 16:39 CET
Plasmid name: pATMx (LMBP 2390)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2390.gb
(View with Genome Compiler) p2390.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Mouse Mx promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pAT153; pSP64Mx |
| Further information: | The plasmid was constructed as follows: the Mx promoter was isolated as a HindIII-EcoRI fragment from pSP64Mx, and ligated between the HindIII and EcoRI sites of the pAT153 vector. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727508.1. |
| EMBL Accession number: | LT727508.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 22/04/1991 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI, EcoRI and HindII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pATMx (LMBP 2390) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vanhoenacker and Prof. Dr G. Haegeman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.