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GeneCorner plasmid details

Last data update: 22 October 2020 04:25 CEST

Plasmid name: pATMxH5HT2R (LMBP 2783)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2783.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human serotonin 5-HT2 receptor (h5HT2R)
Promoter: Mouse Mx promoter
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Mammalian cells; e.g. L929, VERO
Parental clone: pATMxpA2; pUCH5HT2R
Further information: The plasmid was constructed by inserting the NheI-XbaI fragment of pUCH5HT2R, containing the human serotonin 5HT2 receptor gene (H5HT2R), into the unique XbaI site of pATMxpA2.
This plasmid allows the expression of the human serotonin 5HT2 receptor under control of the mouse Mx promoter.
Other name of the plasmid is pATMx5HT2.
EMBL Accession number: -
Latest sequence update: 09/08/1994
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pATMxH5HT2R (LMBP 2783) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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