Last data update: 24 January 2024 16:39 CET
Plasmid name: pATMxH5HT2R (LMBP 2783)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p2783.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human serotonin 5-HT2 receptor (h5HT2R) |
Promoter: | Mouse Mx promoter |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli Mammalian cells; e.g. L929, VERO |
Parental clone: | pATMxpA2; pUCH5HT2R |
Further information: | The plasmid was constructed by inserting the NheI-XbaI fragment of pUCH5HT2R, containing the human serotonin 5HT2 receptor gene (H5HT2R), into the unique XbaI site of pATMxpA2. This plasmid allows the expression of the human serotonin 5HT2 receptor under control of the mouse Mx promoter. Other name of the plasmid is pATMx5HT2. |
EMBL Accession number: | - |
Latest sequence update: | 09/08/1994 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pATMxH5HT2R (LMBP 2783) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.