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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pATMxpA1 (LMBP 2671)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: patmxpa1.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Mouse Mx promoter
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pAT153; pSP64Mx; pSV23S
Further information: The plasmid was constructed as follows : 1) The Mx promoter was isolated as a HindIII-SmaI fragment from pSP64Mx; 2) The SV40 small t- antigen splicing signal and the SV40 polyadenylation signals were isolated as an NcoI (filled in with Klenow) - BamHI fragment from pSV23S; 3) These two fragments were cloned into the HindIII-BamHI opened pAT153 vector, whereby the NcoI site was restored after ligation to the SmaI site
pATMxpA1 contains the SV40 small t-antigen splicing signal.
The Mx promoter is inducible by type I-interferon treatment.
The functionality of the SV40 origin was not experimentally verified.
EMBL Accession number: -
Latest sequence update: 04/02/1992
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pATMxpA1 (LMBP 2671) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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