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GeneCorner plasmid details

Last data update: 26 October 2020 04:25 CET

Plasmid name: pATSVTMx (LMBP 2391)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: patsvtmx.gb (View with Genome Compiler)
patsvtmx.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Simian virus 40 (SV40) gene encoding large T-antigen
Promoter: Mouse Mx promoter
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153; pSP64Mx; pMaSVT
Further information: The plasmid was constructed by ligating the following three fragments: the Mx promoter from pSP64Mx (HindIII-SmaI fragment), SV40 large T-antigen from pMaSVT (StuI-BamHI fragment) and the large BamHI- HindIII fragment from pAT153 (containing the replication origin and the ampicillin gene). The tetracycline gene of pAT153 is lost due to the construction.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727509.1.
EMBL Accession number: LT727509.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 23/04/1991
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglI, EcoRI, HindII and HpaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pATSVTMx (LMBP 2391) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vanhoenacker and Prof. Dr G. Haegeman.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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