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GeneCorner plasmid details

Last data update: 31 October 2020 04:21 CET

Plasmid name: pATTrpMTNFm32Y (LMBP 2698)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse tumor necrosis factor cDNA (Tnf, TNF); mutated sequence
Promoter: Escherichia coli tryptophan operon promoter (trp)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains expressing the trpR gene coding for the tryptophan operon repressor protein (e.g. K514).
Further information: The plasmid contains mutant mTnf; amino acid 32 is replaced by Tyr.
EMBL Accession number: M11731, view at EMBL, GenBank, DDBJ
Latest sequence update: 24/02/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pATTrpMTNFm32Y (LMBP 2698) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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