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GeneCorner plasmid details

Last data update: 25 October 2020 04:11 CET

Plasmid name: pATTrpMTNFm84V (LMBP 2170)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2170.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse tumor necrosis factor cDNA (Tnf, TNF); mature sequence, A84V mutant
Promoter: Escherichia coli tryptophan operon promoter (trp)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains expressing the trpR gene coding for the tryptophan operon repressor protein (e.g. K514).
Parental clone: pATTrpMTNF; pSV23SMTNFm84V
Further information: The plasmid was constructed as follows : the BstXI fragment of pATTrpMTNF was replaced by the corresponding BstXI fragment of pSV23SMTNFm84V.
The plasmid contains mutant MTNF ; amino acid 84 (GCT), coding for Ala is replaced by GTG (coding for Val), and a new Sau3AI restriction site is created.
This mutation is described as A84V, according to the numbering of the codons in the human TNF coding sequence, although the mutated mouse codon is located on position 83.
EMBL Accession number: M11731, view at EMBL, GenBank, DDBJ
Latest sequence update: 17/10/1992
Sequence detail:
mouse TNF wild type:

    80  81  82  83  84  85  86  87
5' GTC AGC CGA TTT GCT ATC TCA TAC 3'
   Val Ser Arg Phe Ala Ile Ser Tyr

mouse TNF mutant:

    80  81  82  83  84  85  86  87
5' GTC AGC CGA TTT GTG ATC TCA TAC 3'
   Val Ser Arg Phe Val Ile Ser Tyr
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed by Dr X. Van Ostade.
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Van Ostade et al., EMBO J. 10 (1991), 827-836 [PMID: 2009860]
Van Ostade et al., Eur. J. Biochem. 220 (1994), 771-779 [PMID: 8143731]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pATTrpMTNFm84V (LMBP 2170) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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