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GeneCorner plasmid details

Last data update: 21 October 2020 04:26 CEST

Plasmid name: pATcE6Hy3mA (LMBP 2314)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2314.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) cDNA; mutated mouse::human chimeric heavy chain (E6Hy3m)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pATcE6Hy3; pBR325OMMBfmA
Further information: The plasmid was constructed by substituting the SacI-MstII fragment from pATcE6Hy3 by the SacI-MstII (nucleotide position 5122) fragment from pBR325OMMBfmA, resulting in an ApaI site followed by a termination codon after the fourth hinge region H4; hence, the CH2 domain and the CH3 domain are no longer part of the coding information.
The chimeric CH1 domain contains 60 amino acids of mouse (E6H) and 37 amino acids of human (hIG3) origin.
Other name of the plasmid is pATcE6Hy3ApaI.
EMBL Accession number: -
Latest sequence update: 22/01/1997
Sequence detail:
Nucleotide sequence at the end of the fourth hinge region of hIG3 (H4):

5' GG TGC CCA GGG CCC TAA CTC TTG GGA GGA CCG 3'
            * +       @
              ApaI

*: End of the fourth hinge region (H4).
+: Start of the CH2 domain of hIG3.
@: Termination codon.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. De Sutter(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pATcE6Hy3mA (LMBP 2314) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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