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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: pATgMIFNG (LMBP 1434)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p1434.gb (View with Genome Compiler)
p1434.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse interferon γ genomic DNA (IFNG)
Promoter: Mouse interferon γ promoter (IFNG)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pAT153
Further information: pATgMIFNG is a pAT153 vector with in the BamHI site a 10.5 kb BamHI fragment of the EMBL 3A-7Mug phage, containing the genomic mouse interferon γ gene (mIFNG).
The orientation of the inserted gene is unknown.
Since the nucleotide sequences of the introns of the genomic mIFNγ gene could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test.
The approximative length of the plasmid and of the mIFNG insert deduced from the compiled nucleotide sequence could be confirmed. Only the 1509 bp PvuI/BglII fragment, containing part of the 3' flanking region of the mIFNG gene, was clearly (+-) 200 bp smaller. Furthermore, the results revealed that EcoRI cuts at least 6 times. The location of the extra EcoRI sites have not been determined; consequently, the compiled nucleotide sequence was not adapted.
Other name of the plasmid is pATgMIFNGB.
EMBL Accession number: -
Latest sequence update: 10/05/1990
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII, BglII/PvuI, EcoRI, HindIII and PvuI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by F. Duerinck(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Gray et al., Proc. Natl. Acad. Sci. U.S.A. 80 (1983), 5842-5846 [PMID: 6310596]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pATgMIFNG (LMBP 1434) is available at BCCM/GeneCorner. This plasmid was deposited by F. Duerinck and Prof. Dr E. Remaut.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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