Last data update: 27 October 2020 04:27 CET
Plasmid name: pATgMIFNG (LMBP 1434)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Mouse interferon γ genomic DNA (IFNG)
|Promoter:||Mouse interferon γ promoter (IFNG)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||pATgMIFNG is a pAT153 vector with in the BamHI site a 10.5 kb BamHI fragment of the EMBL 3A-7Mug phage, containing the genomic mouse interferon γ gene (mIFNG).
The orientation of the inserted gene is unknown.
Since the nucleotide sequences of the introns of the genomic mIFNγ gene could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test.
The approximative length of the plasmid and of the mIFNG insert deduced from the compiled nucleotide sequence could be confirmed. Only the 1509 bp PvuI/BglII fragment, containing part of the 3' flanking region of the mIFNG gene, was clearly (+-) 200 bp smaller. Furthermore, the results revealed that EcoRI cuts at least 6 times. The location of the extra EcoRI sites have not been determined; consequently, the compiled nucleotide sequence was not adapted.
Other name of the plasmid is pATgMIFNGB.
|EMBL Accession number:||-|
|Latest sequence update:||10/05/1990|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII, BglII/PvuI, EcoRI, HindIII and PvuI.|
|History of deposit:||This plasmid was deposited by F. Duerinck(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Related plasmid reference:||Gray et al., Proc. Natl. Acad. Sci. U.S.A. 80 (1983), 5842-5846 [PMID: 6310596]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pATgMIFNG (LMBP 1434) is available at BCCM/GeneCorner. This plasmid was deposited by F. Duerinck and Prof. Dr E. Remaut.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.