Last data update: 27 October 2020 04:27 CET
Plasmid name: pATgMIL2-A (LMBP 1088)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse interleukin 2 genomic DNA (IL2)
|Promoter:||Mouse interleukin 2 promoter (mIL2)|
|Ribosome binding site (RBS) of the mouse interleukin 2 gene (mIL2)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||This plasmid was constructed by inserting an approx. 12.5 kb large SalI fragment (from EMBL3-gMIL2-A) into the unique SalI site of pAT153. The plasmid was BamHI cut to verify the orientation of the inserted fragment.
pAT153 is a pBR322 derivative in which the bom site as well as the region coding for the rop protein have been deleted.
Since the nucleotide sequences of the flanking regions and of large parts of the introns of the mIL2 gene are not known, there is uncertainty as to the cutting frequency of the restriction enzymes indicated.
Other name of the plasmid is pAT153gMIL2A.
|EMBL Accession number:||-|
|Latest sequence update:||26/02/1994|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Degrave et al., Mol. Biol. Rep. 11 (1986), 57-61 [PMID: 3003564]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pATgMIL2-A (LMBP 1088) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Degrave et al., 1986.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.