GREAT AT SMALL THINGS

0

GeneCorner plasmid details

Last data update: 19 October 2020 04:30 CEST

Plasmid name: pATmIL6hIFNG (LMBP 3665)

Add to cart

New search

Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p3665.gb (View with Genome Compiler)
p3665.txt
p3665.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interferon γ cDNA (IFNG)
Promoter: Mouse interleukin 6 promoter (IL6)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Mammalian cells
Parental clone: pATHIL6HIFNG; pBLmIL6CAT
Further information: The plasmid was constructed as follows: the BsaI (Eco31I)-XhoI fragment of pBLmIL6CAT, containing a part of the ampicillin resistance gene and the mouse IL6 promoter, was ligated to the BsaI (Eco31I at position 5299)-XhoI fragment of pATHIL6HIFNG, containing the human IFNγ cDNA, the pMB1 origin and the completing part of the ampicillin resistance gene.
pATmIL6hIFNG contains the SV40 small t-antigen splicing signal.
It also contains three SV40 polyadenylation segments; hence, the possibility might exist that the plasmid could loose information by homologous recombination.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727371.1.
The nucleotide sequence of the genomic mouse IL6 DNA corresponds with the Genbank accession number M20572.1.
EMBL Accession number: M20572.1, view at EMBL, GenBank, DDBJ
LT727371.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/05/2004
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: XbaI and XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr P. Brouckaert(1)(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pATmIL6hIFNG (LMBP 3665) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Brouckaert.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search