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GeneCorner plasmid details

Last data update: 24 October 2020 04:12 CEST

Plasmid name: pAZIL (LMBP 9596)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9596.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli lac Z gene (lacZ); 5' fragment with modified 5' end
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Erythromycin (ery)
Replicon: Escherichia coli plasmid p15A origin
Enterococcus faecalis plasmid pAMβ1 origin, incl. repD and repE
Host range: Escherichia coli
Gram-positive bacterial strains
Parental clone: pUC19; pACYC184; pIL253
Further information: The plasmid was constructed by combining the lacZ gene with multiple cloning site from pUC19, the erythromycin resistance gene and the pAMβ1 origin of replication from pIL253 and the origin of replication from pACYC184.
This plasmid is an E. coli - Lactococcus shuttle cloning vector, allowing the cloning of large DNA fragments (more than 16 kb), blue-white selection of the inserted fragments, and high stability of the constructs.
The nucleotide sequence of the pAZIL vector corresponds with the EMBL Nucleotide Sequence Database accession number FR872379.1, except pAZIL does not contain the cos site (positions 7102-8186).
EMBL Accession number: FR872379.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/08/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. Kojic(1).
(1) Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
Plasmid reference: Kojic et al., BMC Microbiol. 11 (2011), 265 [PMID: 22182285] [DOI: 10.1186/1471-2180-11-265]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + erythromycin (150 µg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pAZIL (LMBP 9596) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Kojic and was published in Kojic et al., 2011.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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