Last data update: 24 January 2024 16:39 CET
Plasmid name: pAZILcos (LMBP 9597)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p9597.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Escherichia coli lac Z gene (lacZ); 5' fragment with modified 5' end |
Promoter: | - |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Erythromycin (ery) |
Replicon: | Escherichia coli plasmid p15A origin Enterococcus faecalis plasmid pAMβ1 origin, incl. repD and repE |
Copy number: | Low copy number |
Host range: | Escherichia coli Gram-positive bacterial strains |
Parental clone: | pSuperCos; pAZIL |
Further information: | The plasmid was constructed by cloning the cos site from pSuperCos into the SacII opened pAZIL vector. This cosmid is an E. coli - Lactococcus shuttle cloning vector, allowing the cloning of large DNA fragments, blue-white selection of the inserted fragments, and high stability of the constructs. The nucleotide sequence of the pAZIL vector corresponds with the EMBL Nucleotide Sequence Database accession number FR872379.1. |
EMBL Accession number: | FR872379.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 03/08/2015 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr M. Kojic(1). (1) Laboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia |
Plasmid reference: | - |
Related plasmid reference: | Kojic et al., BMC Microbiol. 11 (2011), 265 [PMID: 22182285] [DOI: 10.1186/1471-2180-11-265] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + erythromycin (150 µg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pAZILcos (LMBP 9597) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Kojic. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.