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GeneCorner plasmid details

Last data update: 23 October 2020 04:27 CEST

Plasmid name: pAqpZ-CF (LMBP 11184)

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Price category: Cat. 3 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p11184.gb
Sequence
analysis results
Genecorner:

NGS: h05-gc-dec2018.fasta

Cloned DNA: Escherichia coli water channel AqpZ cDNA (aqpZ, aquaporin Z, bniP, GeneID 945497)
Histidine tag (His-tag); C-terminal
Promoter: Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Phage T7 gene 10 terminator (T7g10)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pEXP5-CT/TOPO
Further information: The plasmid was constructed by cloning the E. coli aqpZ coding sequence into the pEXP5-CT/TOPO vector.
The nucleotide sequence of the E. coli aqpZ coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number U38664.1, except for the silent nucleotide changes at positions 3 (G->C) and 294 (C->G) and the nucleotide change at position 295 (G->C) leading to a V99L codon change.
Other name of the plasmid is pEXP5-ecAQPZ-His.
EMBL Accession number: U38664.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 14/03/2019
Authenticity test: This plasmid has been fully sequenced.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Dr G. Calamita (1). It was constructed by D. Frascaria (1).
(1) Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari "Aldo Moro", Bari, Italy
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pAqpZ-CF (LMBP 11184) is available at BCCM/GeneCorner. The plasmid was deposited by Dr G. Calamita .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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