Last data update: 16 April 2021 10:31 CEST
Plasmid name: pB2H-delta-omega (LMBP 6701)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
Escherichia coli lac repressor gene (lacI)|
Escherichia coli lac Z gene (lacZ); fragment lacking codons 788-1023 (lacZΔω)
|Promoter:||Phage SP6 promoter
Phage T7 gene 10 promoter (T7g10)
Escherichia coli lac operon promoter
Escherichia coli lac repressor promoter (lacI)
Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)
|Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)|
|Terminator:||Phage T7 gene 10 terminator (T7g10)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli|
|Further information:||The plasmid was created by cloning the E. coli lacZΔω fragment as a BamHI-NcoI fragment into the pETDuet-1ΔSphIΩtac vector. This parental vector was created by inserting the tac promoter as an EcoRV-PstI fragment from pALTER-Ex1, after deleting the BamHI restriction site, in the pETDuet-1 plasmid with the SphI site deleted.
The plasmid can be used in bacterial two-hybrid systems which are based on trans complementation of β-galactosidase (e.g. by co-expression with pB2H-delta-alfa in lacZ(-) strains).
The T7 terminator is not functional for this construct.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726841.1.
Both the 5' and the 3' end of the E. coli lacZΔω fragment were sequenced by BCCM/GeneCorner. The length of the plasmid described in the sequence file that BCCM/GeneCorner obtained from the depositor does not correspond to the length described in Borloo et al. (2007). Furthermore, the sequence file also lacks codon 788 of lacZ, in contrary to Borloo et al. (2007).
Name mentioned in Borloo et al. (2007) is pB2HΔω.
|EMBL Accession number:||LT726841.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||15/06/2010|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI, BamHI/NcoI, HincII, HindIII, NcoI, SacI and SphI.|
|History of deposit:||This plasmid was deposited by Prof. Dr B. Devreese(1). It was constructed by J. Borloo(1).
(1) Laboratory for Protein Biochemistry and Protein Engineering, Department of Biochemistry, Physiology and Microbiology, Ghent University, Belgium
|Plasmid reference:||Borloo et al., J. Proteome Res. 6 (2007), 2587-2595 [PMID: 17539672]
|Related plasmid reference:||Mayola et al., PLoS ONE 22 (2014), e105578 [PMID: 25147953] [DOI: 10.1371/journal.pone.0105578]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pB2H-delta-omega (LMBP 6701) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr B. Devreese and was published in Borloo et al., 2007.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.