Last data update: 24 January 2024 16:39 CET
Plasmid name: pBAD24-ProP-Y44F (LMBP 12184)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli osmolyte:H+ symporter ProP cDNA (proP, GeneID 948626); mutated sequence Escherichia coli DNA-binding transcriptional dual regulator AraC cDNA (araC, GeneID 944780) |
| Promoter: | Escherichia coli L-arabinose operon promoter (araB, araBAD, BAD) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBAD24-ProP |
| Further information: | The plasmid was constructed by introducing a Y44F mutation into the E. coli ProP coding sequence of pBAD24-ProP. The nucleotide sequence of the wild type E. coli ProP cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M83089.1. The nucleotide sequence of the parental pBAD24 vector corresponds with the EMBL Nucleotide Sequence Database accession number X81837.1. Other name of the plasmid is pDC269. |
| EMBL Accession number: | M83089.1, view at EMBL, GenBank, DDBJ X81837.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/10/2020 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr D.E. Culham(1) and Prof. Em. Dr J.M. Wood(1). (1) Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada |
| Plasmid reference: | Keates et al., Biochemistry 49 (2010), 8847-8856 [PMID: 20828170] [DOI: 10.1021/bi101281f] |
| Related plasmid reference: | Culham et al., J. Mol. Recognit. 13 (2000), 309-322 [PMID: 10992293] [DOI: 10.1002/1099-1352(200009/10)13:5<309::AID-JMR505>3.0.CO;2-R] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 WG350 (LMBP 12428) |
| Host reference: | Culham et al., J. Mol. Biol. 229 (1993), 268-276 [PMID: 8421314] [DOI: 10.1006/jmbi.1993.1030] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBAD24-ProP-Y44F (LMBP 12184) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Keates et al., 2010. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.