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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: pBAD24-ProP-Y44F (LMBP 12184)

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Price category: Cat. 1 (cf. price list)
Status:
Depositor's sequence: -
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli osmolyte:H(+) symporter ProP cDNA (proP, GeneID 948626); mutated sequence
Escherichia coli DNA-binding transcriptional dual regulator AraC cDNA (araC, GeneID 944780)
Promoter: Escherichia coli araBAD promoter (PBAD)
Ribosome
binding site:
-
Terminator: Escherichia coli rrnB operon T1 terminator
Escherichia coli rrnB operon T2 terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pBAD24-ProP
Further information: The plasmid was constructed by introducing a Y44F mutation into the E. coli ProP coding sequence of pBAD24-ProP.
The nucleotide sequence of the wild type E. coli ProP cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M83089.1.
Other name of the plasmid is pDC269.
EMBL Accession number: M83089.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 09/10/2020
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This host strain was deposited by Dr D.E. Culham(1) and Prof. Em. Dr J.M. Wood(1).
(1) Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada
Plasmid reference: Keates et al., Biochemistry 49 (2010), 8847-8856 [PMID: 20828170] [DOI: 10.1021/bi101281f]
Related plasmid reference: Culham et al., J. Mol. Recognit. 13 (2000), 309-322 [PMID: 10992293] [DOI: 10.1002/1099-1352(200009/10)13:5<309::AID-JMR505>3.0.CO;2-R]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 WG350
Host reference: Culham et al., J. Mol. Biol. 229 (1993), 268-276 [PMID: 8421314] [DOI: 10.1006/jmbi.1993.1030]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBAD24-ProP-Y44F (LMBP 12184) is available at BCCM/GeneCorner. This host strain was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Keates et al., 2010.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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