Last data update: 27 October 2020 04:27 CET
Plasmid name: pBAD24-ProP-Y44F (LMBP 12184)
|Price category:||Cat. 1 (cf. price list)|
|Cloned DNA:||Escherichia coli osmolyte:H(+) symporter ProP cDNA (proP, GeneID 948626); mutated sequence
Escherichia coli DNA-binding transcriptional dual regulator AraC cDNA (araC, GeneID 944780)
|Promoter:||Escherichia coli araBAD promoter (PBAD)|
|Terminator:||Escherichia coli rrnB operon T1 terminator
Escherichia coli rrnB operon T2 terminator
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli|
|Further information:||The plasmid was constructed by introducing a Y44F mutation into the E. coli ProP coding sequence of pBAD24-ProP.
The nucleotide sequence of the wild type E. coli ProP cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M83089.1.
Other name of the plasmid is pDC269.
|EMBL Accession number:||M83089.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||09/10/2020|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This host strain was deposited by Dr D.E. Culham(1) and Prof. Em. Dr J.M. Wood(1).
(1) Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada
|Plasmid reference:||Keates et al., Biochemistry 49 (2010), 8847-8856 [PMID: 20828170] [DOI: 10.1021/bi101281f]
|Related plasmid reference:||Culham et al., J. Mol. Recognit. 13 (2000), 309-322 [PMID: 10992293] [DOI: 10.1002/1099-1352(200009/10)13:5<309::AID-JMR505>3.0.CO;2-R]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 WG350|
|Host reference:||Culham et al., J. Mol. Biol. 229 (1993), 268-276 [PMID: 8421314] [DOI: 10.1006/jmbi.1993.1030]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBAD24-ProP-Y44F (LMBP 12184) is available at BCCM/GeneCorner. This host strain was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Keates et al., 2010.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.