Last data update: 24 January 2024 16:39 CET
Plasmid name: pBARGPE1 (LMBP 8049)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8049.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter (gpdA) Aspergillus nidulans tryptophan C promoter (trpC) |
| Ribosome binding site: |
- |
| Terminator: | Aspergillus nidulans tryptophan C terminator (trpC) |
| Selection marker: | Ampicillin (amp) Bialaphos (bar; basta) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Fungi |
| Parental clone: | - |
| Further information: | pBARGPE1 is a fungal expression vector designed to express genes or cDNAs under their own initiation codons. The plasmid carries a lox-NotI-lox sequence for insertion into lambda and for site-specific recombination between lox sites, catalyzed by the Cre protein. The plasmid also carries the Streptomyces hygroscopicus bar gene conferring resistance to the herbicide bialaphos. The nucleotide sequence of the bar gene corresponds with the EMBL Nucleotide Sequence Database accession number X05822.1. The nucleotide sequence of the gdpA promoter corresponds with the EMBL Nucleotide Sequence Database accession number M33539.1. The nucleotide sequences of the trpC promoter and terminator correspond with the EMBL Nucleotide Sequence Database accession number X02390.1. Since the nucleotide sequence of the pBARGPE1 vector could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test. |
| EMBL Accession number: | X05822.1, view at EMBL, GenBank, DDBJ M33539.1, view at EMBL, GenBank, DDBJ X02390.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 14/01/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRV/ScaI, NdeI/XbaI and NsiI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr K. McCluskey(1). (1) FGSC, University of Missouri, Kansas City, USA |
| Plasmid reference: | Pall et al., Fungal Genet. Newsl. 40 (1993), 59-62 |
| Related plasmid reference: | Pall et al., Fungal Genet. Newsl. 41 (1994), 63-65 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBARGPE1 (LMBP 8049) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Pall et al., 1993. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.