Last data update: 30 October 2020 04:11 CET
Plasmid name: pBC-Phleo (LMBP 8051)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Escherichia coli lac Z gene (lacZ); fragment including α part
|Promoter:||Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter (gpdA)
Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
|Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)|
|Terminator:||Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)|
|Selection marker:||Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo))
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli
|Parental clone:||pBC-SK+; pUT703|
|Further information:||The plasmid was created by cloning the phleomycin resistance cassette from pUT703 as an EcoRI/HindIII fragment into the pBC SK+ vector.
Name used in Silar et al. (1995) is pBC-phleo.
The nucleotide sequence of the gdpA promoter corresponds with the EMBL Nucleotide Sequence Database accession number M33539.1.
Since the nucleotide sequence of the pBC-Phleo vector could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test.
|EMBL Accession number:||M33539.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||14/01/2013|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: EcoRI, PvuII and SspI.|
|History of deposit:||This plasmid was deposited by Prof. Dr K. McCluskey(1).
(1) FGSC, University of Missouri, Kansas City, USA
|Plasmid reference:||Silar, Fungal Genet. Newsl. 42 (1995), 73
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
|Cultivation medium:||LB-Lennox + chloramphenicol (34 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBC-Phleo (LMBP 8051) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Silar, 1995.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.