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GeneCorner plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pBC18R (LMBP 9303)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Phage T7 gene 10 promoter (T7g10)
Escherichia coli lac operon promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Broad-host-range plasmid RK2 origin of transfer (oriT)
Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Gram-negative bacterial strains
Parental clone: pZT18R; pBC19R
Further information: The plasmid was constructed by replacing the 346 bp PvuII fragment containing the MCS of pBC19R with the MCS of pTZ18R.
pBC18R is a mobilisable broad-host-range cloning vector.
Other name of the plasmid is pTZ18R oriTRK2.
EMBL Accession number: -
Latest sequence update: 03/07/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr B. China(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Unit of Microbiology, Catholic University of Louvain, Brussels, Belgium.
Plasmid reference: China et al., Mol. Microbiol. 4 (1990), 1585-1593 [PMID: 2287280]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 S17-1
Host reference: -
Related host reference: Simon et al., Biotechnology 1 (1983), 784-791
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBC18R (LMBP 9303) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in China et al., 1990.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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