Last data update: 24 January 2024 16:39 CET
Plasmid name: pBIC (LMBP 3450)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3450.gb
(View with Genome Compiler) p3450.txt p3450.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Photinus pyralis (firefly) luciferase gene (LUC) Chloramphenicol acetyltransferase gene (cat) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) Herpes simplex virus (HSV) thymidine kinase promoter (TK) |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the C-S8c1 foot and mouth disease virus polyprotein gene (FMDV-POLYP) |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pGEM2 |
| Further information: | pBIC is an expression vector for bicistronic mRNA's. Translation of the second gene, i.e. the firefly luciferase gene, is promoted by the internal ribosome entry site of the C-S8c1 foot and mouth disease virus polyprotein gene. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727339.1. The nucleotide sequence of this plasmid was compiled according to Martinez-Salas et al. (1993). The nucleotide sequence of the fragment containing the C-S8c1 FMDV-POLYP IRES was provided by Dr E. Martinez-Salas. The nucleotide sequence of the P. pyralis LUC cds and 3' UTR correspond with the EMBL Nucleotide Sequence Database accession number M15077.1. The nucleotide sequence of the HSV-TK promoter corresponds with the EMBL Nucleotide Sequence Database accession number V00470.1. The nucleotide sequence of the pGEM2 vector corresponds with the EMBL Nucleotide Sequence Database accession number L08871.1 and adapted to the sequence analysis of Dr Martin Mokrejs (Faculty of Science, Charles University, Prague, Czech Republic). |
| EMBL Accession number: | V00470.1, view at EMBL, GenBank, DDBJ L08871.1, view at EMBL, GenBank, DDBJ M15077.1, view at EMBL, GenBank, DDBJ LT727339.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 13/03/2009 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AlwNI/NcoI, BamHI, Bsp119I/Eco130I, Eco47III/Psp5II, Eco88I/PstI, HindIII, HindIII/SphI, PvuII/TatI, SacI/SspI and XhoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr E. Martinez-Salas(1). (1) Universidad Autonoma de Madrid, Madrid, Spain |
| Plasmid reference: | Martinez-Salas et al., J. Virol. 67 (1993), 3748-3755 [PMID: 8389904] |
| Related plasmid reference: | Martinez-Salas et al., Genes Dev. 3 (1989), 1493-1506 [PMID: 2558965] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
| Related website: | http://www.iresite.org/IRESite_web.php?page=view&entry_id=321 |
Refer in your Materials and Methods: |
pBIC (LMBP 3450) is available at BCCM/GeneCorner. This plasmid was deposited by Dr E. Martinez-Salas and was published in Martinez-Salas et al., 1993. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.