GeneCorner plasmid details

Last data update: 30 July 2021 10:22 CEST

Plasmid name: pBK-1 (LMBP 4707)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: (View with Genome Compiler)
analysis results


Cloned DNA: BK virus DNA (BKV); fragment of the Gardner strain
Promoter: BK virus early promoter (BKV early)
BK virus late promoter (BKV late)
binding site:
Terminator: BK virus polyadenylation signal (BKV polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
BK virus origin (BKV)
Host range: Escherichia coli
Mammalian cells
Parental clone: pML
Further information: pBK-1 was constructed by ligating the 5.1 kb BamHI-EcoRI fragment from the BK virus (BKV; Gardner strain) to the 2.6 kb BamHI-EcoRI fragment from pML. As a result, the BKV late genes were disrupted.
pML (or pML-I) is a pBR322 derivative with deletion of the poison sequences (from pBR322 position 1094 to 2483).
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727166.1.
EMBL Accession number: LT727166.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 09/06/1994
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI/EcoRI, EcoRI and ScaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Barbanti-Brodano(1).
(1) Institute of Microbiology, School of Medicine, University of Ferrara, Italy
Plasmid reference: Milanesi et al., Mol. Cell. Biol. 4 (1984), 1551-1560 [PMID: 6092918]
Related plasmid reference: Lusky et al., Nature 293 (1981), 79-81 [PMID: 6267479]
Grossi et al., Arch. Virol. 99 (1988), 249-259 [PMID: 2835949]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBK-1 (LMBP 4707) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Barbanti-Brodano and was published in Milanesi et al., 1984.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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