Last data update: 31 October 2020 04:21 CET
Plasmid name: pBLCAT5 (LMBP 2451)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Chloramphenicol acetyltransferase gene (cat)
|Promoter:||Herpes simplex virus (HSV) thymidine kinase promoter (TK)
Escherichia coli lac operon promoter
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; preferably recombination-deficient strains
|Further information:||This cat reporter plasmid was constructed by deleting 467 nucleotides of the vector sequence immediately upstream of the polylinker from pBLCAT2 (Luckow et al. (1987)) and replacing it by two fragments from SV40 DNA, inserted in tandem and each containing the polyadenylation signal of the large T-antigen.
pBLCAT5 contains the SV40 small t-antigen splicing signal.
This plasmid contains three SV40 polyA segments; hence, the possibility might exist that the plasmid could loose information by homologous recombination; so far, no observations have been made in this respect (Boshart, personal communication).
pBLCAT5 allows functional analysis of the eukaryotic HSV thymidine kinase promoter.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727516.1.
|EMBL Accession number:||LT727516.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||10/10/2003|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindII, HindIII and RsaI.|
|History of deposit:||This plasmid was deposited by Dr G. Schütz(1).
(1) German Cancer Research Center, Heidelberg, Germany
|Plasmid reference:||Boshart et al., Gene 110 (1992), 129-130 [PMID: 1544570]
|Related plasmid reference:||Luckow et al., Nucleic Acids Res. 15 (1987), 5490 [PMID: 3037497]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBLCAT5 (LMBP 2451) is available at BCCM/GeneCorner. This plasmid was deposited by Dr G. Schütz and was published in Boshart et al., 1992.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.