Last data update: 24 January 2024 16:39 CET
Plasmid name: pBLCAT5HIL6305Tk (LMBP 3179)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human interleukin 6 genomic DNA (IL6, IL-6, IFNB2, BSF2, GeneID 3569) |
Promoter: | Herpes simplex virus (HSV) thymidine kinase promoter (TK); partial |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) Chloramphenicol (cam) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pBLHIL6CAT; pBLCAT5Tk |
Further information: | The plasmid was constructed by ligating the 305 bp NspHI (blunted)-XhoI fragment from pBLHIL6CAT into the BamHI (blunted)-XhoI opened the pBLCAT5Tk vector. The nucleotide sequence of the genomic human IL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11. |
EMBL Accession number: | AC073072.11, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 20/08/2013 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBLCAT5HIL6305Tk (LMBP 3179) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.