Last data update: 14 May 2021 11:30 CEST
Plasmid name: pBLCAT6 (LMBP 2452)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
Chloramphenicol acetyltransferase gene (cat)|
|Promoter:||Escherichia coli lac operon promoter|
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; preferably recombination-deficient strains
|Further information:||This cat reporter plasmid was constructed by deleting 467 bp of vector sequence immediately upstream of the polylinker from pBLCAT3 (Luckow et al., 1987), and replacing it by two fragments from SV40 DNA, inserted in tandem and each containing the SV40 polyadenylation signal.
pBLCAT6 contains the SV40 small t-antigen splicing signal.
This plasmid contains three SV40 polyA segments; hence, the possibility might exist that the plasmid could lose information by homologous recombination; so far, no observations have been made in this respect (Boshart, personal communication).
pBLCAT6 is most useful for insertion of an eukaryotic promoter sequence and determination of the corresponding expression efficiency under various conditions.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727517.1.
|EMBL Accession number:||LT727517.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||15/10/2003|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindII, HindIII, PstI and RsaI.|
|History of deposit:||This plasmid was deposited by Dr G. Schütz(1).
(1) German Cancer Research Center, Heidelberg
|Plasmid reference:||Boshart et al., Gene 110 (1992), 129-130 [PMID: 1544570]
|Related plasmid reference:||Luckow et al., Nucleic Acids Res. 15 (1987), 5490 [PMID: 3037497]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBLCAT6 (LMBP 2452) is available at BCCM/GeneCorner. This plasmid was deposited by Dr G. Schütz and was published in Boshart et al., 1992.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.