Last data update: 24 January 2024 16:39 CET
Plasmid name: pBLF58-1 (LMBP 2776)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human TNF receptor superfamily, member 6 cDNA (FAS) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) Phage T3 promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | - |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pCEV4 |
| Further information: | The plasmid was constructed by inserting a 2.6 kb fragment, containing the human Fas antigen cDNA, into pCEV4. The nucleotide sequence of the human Fas antigen cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M67454.1. |
| EMBL Accession number: | M67454.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 21/01/1997 |
| Authenticity test: | By lack of sequence data, it was not possible to perform the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr S. Nagata(1). (1) University Medical School, Osaka, Japan |
| Plasmid reference: | - |
| Related plasmid reference: | Itoh et al., Cell 66 (1991), 233-243 [PMID: 1713127] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBLF58-1 (LMBP 2776) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Nagata. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.