GeneCorner plasmid details

Last data update: 17 June 2021 09:10 CEST

Plasmid name: pBLF58-1 (LMBP 2776)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
analysis results


Cloned DNA: Human TNF receptor superfamily, member 6 cDNA (FAS)
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
binding site:
Terminator: -
Selection marker: -
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pCEV4
Further information: The plasmid was constructed by inserting a 2.6 kb fragment, containing the human Fas antigen cDNA, into pCEV4.
The nucleotide sequence of the human Fas antigen cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M67454.1.
EMBL Accession number: M67454.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 21/01/1997
Authenticity test: By lack of sequence data, it was not possible to perform the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Nagata(1).
(1) University Medical School, Osaka, Japan
Plasmid reference: -
Related plasmid reference: Itoh et al., Cell 66 (1991), 233-243 [PMID: 1713127]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBLF58-1 (LMBP 2776) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Nagata.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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