Last data update: 21 October 2021 10:13 CEST
Plasmid name: pBLHIL6CAT (LMBP 2861)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
Chloramphenicol acetyltransferase gene (cat)|
|Promoter:||Human interleukin 6 promoter (IL6)
Escherichia coli lac operon promoter
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; preferably recombination-deficient strains
|Parental clone:||pBLCAT5; pGEM1gHIL61|
|Further information:||The plasmid was constructed by replacing the BamHI-XhoI fragment, containing the HSV-TK promoter, of pBLCAT5 by the 1186 bp BamHI-XhoI fragment, containing the hIL6 promoter, of pGEM1gHIL61.
The resulting plasmid allows functional analysis of the human IL6 promoter.
This plasmid contains the SV40 small t-antigen splicing signal. It also contains three SV40 polyA segments. Hence, the possibility might exist that the plasmid could loose information by homologous recombination. So far, no observations have been made in this respect.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727549.1.
The nucleotide sequence of the genomic hIL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11. However, the C nucleotide at promoter position -174 (polymorphism mutant) was changed into a G nucleotide (wild type).
Other names of the plasmid are pBLCAT5HIL6 and pBLHIL6CAT5.
|EMBL Accession number:||AC073072.11, view at EMBL, GenBank, DDBJ
LT727549.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||29/04/2004|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI/XhoI, HindIII, NheI, SphI, SspI and StyI.|
|History of deposit:||This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBLHIL6CAT (LMBP 2861) is available at BCCM/GeneCorner. This plasmid was deposited by Dr W. Vanden Berghe and Prof. Dr G. Haegeman.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.