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GeneCorner plasmid details

Last data update: 22 October 2020 04:25 CEST

Plasmid name: pBLHIL6TK2CAT (LMBP 2987)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 6 genomic DNA (IL6)
Promoter: Human interleukin 6 promoter (IL6)-Herpes simplex virus (HSV) thymidine kinase promoter (TK) hybrid
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBLHIL6CAT; pBLCAT5
Further information: The plasmid was created by isolating the human IL6 cDNA as a BamHI-SspI fragment from pBLHIL6CAT and ligating it into the BstBI (filled in)-BamHI opened pBLCAT5 vector.
The nucleotide sequence of the genomic human IL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11.
EMBL Accession number: AC073072.11, view at EMBL, GenBank, DDBJ
Latest sequence update: 20/08/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBLHIL6TK2CAT (LMBP 2987) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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