Last data update: 22 October 2020 04:25 CEST
Plasmid name: pBLmIL6CAT (LMBP 2988)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Chloramphenicol acetyltransferase gene (cat)
|Promoter:||Mouse interleukin 6 promoter (IL6)
Escherichia coli lac operon promoter
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; preferably recombination-deficient strains
|Parental clone:||pUC18gMIL6; pBLCAT5|
|Further information:||The plasmid was constructed as follows: the BamHI-BanII (blunted with T4 DNA exonuclease) fragment of pUC18gMIL6, containing the mouse IL6 promoter, was inserted between the BamHI-BglII (filled-in with Klenow DNA polymerase) sites of pBLCAT5, hereby replacing the TK promoter.
pBLmIL6CAT contains the SV40 small t-antigen splicing signal.
pBLmIL6CAT contains also three SV40 polyadenylation segments. Hence, the possibility might exist that the plasmid could loose information by homologous recombination.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727418.1.
The nucleotide sequence of the genomic mouse interleukin 6 DNA corresponds with the Genbank accession number M20572.1.
|EMBL Accession number:||M20572.1, view at EMBL, GenBank, DDBJ
LT727418.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||13/10/2003|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI/BanII, EcoRI/PstI, HindII, HindIII, PstI and RsaI.|
|History of deposit:||This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBLmIL6CAT (LMBP 2988) is available at BCCM/GeneCorner. This plasmid was deposited by Dr W. Vanden Berghe and Prof. Dr G. Haegeman.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.