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GeneCorner plasmid details

Last data update: 22 October 2020 04:25 CEST

Plasmid name: pBLmIL6CAT (LMBP 2988)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2988.gb (View with Genome Compiler)
p2988.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Chloramphenicol acetyltransferase gene (cat)
Promoter: Mouse interleukin 6 promoter (IL6)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; preferably recombination-deficient strains
Mammalian cells
Parental clone: pUC18gMIL6; pBLCAT5
Further information: The plasmid was constructed as follows: the BamHI-BanII (blunted with T4 DNA exonuclease) fragment of pUC18gMIL6, containing the mouse IL6 promoter, was inserted between the BamHI-BglII (filled-in with Klenow DNA polymerase) sites of pBLCAT5, hereby replacing the TK promoter.
pBLmIL6CAT contains the SV40 small t-antigen splicing signal.
pBLmIL6CAT contains also three SV40 polyadenylation segments. Hence, the possibility might exist that the plasmid could loose information by homologous recombination.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727418.1.
The nucleotide sequence of the genomic mouse interleukin 6 DNA corresponds with the Genbank accession number M20572.1.
EMBL Accession number: M20572.1, view at EMBL, GenBank, DDBJ
LT727418.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 13/10/2003
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI/BanII, EcoRI/PstI, HindII, HindIII, PstI and RsaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBLmIL6CAT (LMBP 2988) is available at BCCM/GeneCorner. This plasmid was deposited by Dr W. Vanden Berghe and Prof. Dr G. Haegeman.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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