Last data update: 20 April 2021 09:48 CEST
Plasmid name: pBM6DraA6 (LMBP 2207)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
Mouse interleukin 6 cDNA (Il6, Il-6, GeneID 16193)|
|Promoter:||Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Phage SP6 promoter
Phage T7 gene 10 promoter (T7g10)
Rous sarcoma virus long terminal repeat (RSV-LTR)
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Neomycin (neo; G418; kanamycin (kan))
Suppressor tRNA gene (supF; requires a p3 containing host)
|Replicon:||Bacteriophage M13 origin
Escherichia coli plasmid pMB1 origin
Polyoma virus origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli; use strains with the p3 helper plasmid (kan resistant, amber codon in amp and tet)
Mammalian cells; SV40 permissive cells
|Parental clone:||pBM5Neo; pB6Delta2|
|Further information:||This plasmid contains the mouse interleukin 6 (mIL6) coding sequence in the sense orientation relative to the human cytomegalovirus immediate early promoter (CMV-IE) and enhancer.
The plasmid contains the neomycin resistance gene (confers kanamycin resistance in bacteria), which is expressed under the control of the Rous sarcoma virus long terminal repeat (RSV-LTR) as the promoter.
The supF tRNA suppressor gene is used for selection in E. coli MC1061[p3]. It is recommended to select on both ampicillin and tetracycline containing media.
The sequence of the RSV-LTR region corresponds with the Genbank accession number J02025 (version GI:210255).
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727632.1.
The nucleotide sequence of the mIL6 cDNA corresponds with the Genbank accession number X06203.1.
The nucleotide sequence at the 5' end of the mIL6 insert into the EcoRV cleaved pBM5Neo vector is not exactly known.
|EMBL Accession number:||X06203.1, view at EMBL, GenBank, DDBJ
LT727632.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||11/06/1991|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BanII, BglII, BglII/HindII, KpnI, NcoI, PstI, XbaI and XhoI.
As compared to the fragments to be expected from the compiled nucleotide sequence, the 2011 bp BglII fragment seems to be approximately 1800-1900 bp long (located in the agarose gel beneath the 2000 bp fragment of the SMART ladder). Furthermore, it seems that the 2927 bp BanII fragment is splitted into two fragments of approximately 1200 bp and 1700 bp respectively.
|History of deposit:||This plasmid was deposited by Dr J. Van Snick(1).
(1) Ludwig Institute, Brussels, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061[p3]|
|Related host reference:||Invitrogen Instruction Manual
Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Cultivation medium:||LB-Lennox + ampicillin (50 μg/ml) + kanamycin (50 μg/ml) + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBM6DraA6 (LMBP 2207) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Van Snick.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.