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GeneCorner plasmid details

Last data update: 02 December 2020 04:29 CET

Plasmid name: pBMGNeoE6Hy3 (LMBP 1973)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1973.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) half genomic gene; mouse::human chimeric heavy chain (E6Hy3)
Promoter: Mouse metallothionein 1 promoter (Mt1)
Herpes simplex virus (HSV) thymidine kinase promoter (TK)
Ribosome
binding site:
-
Terminator: Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Rabbit β-globin polyadenylation signal (β-globin polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418; kanamycin (kan))
Replicon: Escherichia coli plasmid pMB1 origin
Bovine papilloma virus origin (BPV)
Host range: Escherichia coli
Mammalian cells
Parental clone: pBMGNeo; pSV23E6Hy3
Further information: The plasmid was constructed as follows: 1) After digesting pSV23E6Hy3 with HindIII, the 5' sticky ends were filled in with Klenow and SalI linkers ('CGTCGACG') were ligated; 2) The SalI fragment, containing the E6Hy3 gene, was then cloned into the unique SalI site of pBMGNeo, sense relative to the mMt1 promoter.
The mMt1 promoter controls the expression of the inserted E6Hy3 gene in mammalian cells.
Because of the presence of the second intron of the rabbit β-globin gene in the 3' untranslated region, the E6Hy3 transcript is spliced.
See also pBMGNeo for other remarks.
EMBL Accession number: -
Latest sequence update: 17/06/1991
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Veronique Feys
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB HB101
Host reference: Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
Related host reference: Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBMGNeoE6Hy3 (LMBP 1973) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers and was published in Veronique Feys, .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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