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GeneCorner plasmid details

Last data update: 26 October 2020 04:25 CET

Plasmid name: pBMGNeoE6L (LMBP 1972)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1972.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; light chain (E6L)
Promoter: Mouse metallothionein 1 promoter (Mt1)
Herpes simplex virus (HSV) thymidine kinase promoter (TK)
Ribosome
binding site:
-
Terminator: Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Rabbit β-globin polyadenylation signal (β-globin polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418; kanamycin (kan))
Replicon: Escherichia coli plasmid pMB1 origin
Bovine papilloma virus origin (BPV)
Host range: Escherichia coli
Mammalian cells
Parental clone: pBMGNeo; pSV23SE6L
Further information: The plasmid was constructed as follows : 1) After digesting pSV23SE6L with HindIII, the 5' sticky ends were filled in with Klenow polymerase and SalI linkers ('CGTCGACG') were ligated; 2) The SalI fragment, containing the gene encoding the light chain E6L of the mouse monoclonal antibody E6(IgG2b,κ), was then cloned into the unique SalI site of pBMGNeo, clockwise behind the mMt1 promoter.
The mMt1 promoter controls the expression of the inserted E6L gene in mammalian cells.
Because of the presence of the second intron of the rabbit β-globin gene in the 3' untranslated region, the E6L transcript is spliced.
The 3' untranslated region of the E6L gene still contains 15 dC-nucleotides of the dG/dC tail derived from the cDNA synthesis of E6L (De Waele et al. (1988)).
EMBL Accession number: -
Latest sequence update: 17/06/1991
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Veronique Feys
Related plasmid reference: De Waele et al., Eur. J. Biochem. 176 (1988), 287-295 [PMID: 3138116]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB HB101
Host reference: Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
Related host reference: Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBMGNeoE6L (LMBP 1972) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers and was published in Veronique Feys, .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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