Last data update: 26 October 2020 04:25 CET
Plasmid name: pBMGNeoE6L (LMBP 1972)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; light chain (E6L)
|Promoter:||Mouse metallothionein 1 promoter (Mt1)
Herpes simplex virus (HSV) thymidine kinase promoter (TK)
|Terminator:||Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Rabbit β-globin polyadenylation signal (β-globin polyA)
|Selection marker:||Ampicillin (amp)
Neomycin (neo; G418; kanamycin (kan))
|Replicon:||Escherichia coli plasmid pMB1 origin
Bovine papilloma virus origin (BPV)
|Host range:||Escherichia coli
|Parental clone:||pBMGNeo; pSV23SE6L|
|Further information:||The plasmid was constructed as follows : 1) After digesting pSV23SE6L with HindIII, the 5' sticky ends were filled in with Klenow polymerase and SalI linkers ('CGTCGACG') were ligated; 2) The SalI fragment, containing the gene encoding the light chain E6L of the mouse monoclonal antibody E6(IgG2b,κ), was then cloned into the unique SalI site of pBMGNeo, clockwise behind the mMt1 promoter.
The mMt1 promoter controls the expression of the inserted E6L gene in mammalian cells.
Because of the presence of the second intron of the rabbit β-globin gene in the 3' untranslated region, the E6L transcript is spliced.
The 3' untranslated region of the E6L gene still contains 15 dC-nucleotides of the dG/dC tail derived from the cDNA synthesis of E6L (De Waele et al. (1988)).
|EMBL Accession number:||-|
|Latest sequence update:||17/06/1991|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||PhD thesis Veronique Feys
|Related plasmid reference:||De Waele et al., Eur. J. Biochem. 176 (1988), 287-295 [PMID: 3138116]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12xB HB101|
|Host reference:||Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
|Related host reference:||Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBMGNeoE6L (LMBP 1972) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers and was published in Veronique Feys, .|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.