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GeneCorner plasmid details

Last data update: 04 December 2020 04:17 CET

Plasmid name: pBR322 (LMBP 140)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p140.gb (View with Genome Compiler)
p140.txt
p140.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Further information: HindIII does not always inactivate the tetracycline resistance gene.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number J01749 .1.
EMBL Accession number: J01749.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 23/01/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, HindII, HindIII/PstI and RsaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr F. Bolivar(1).
(1) Universidad Nacional Autonoma de Mexico, Institute of Biotechnology, Cuernavaca, Morelos, Mexico
Plasmid reference: Bolivar et al., Gene 2 (1977), 95-113 [PMID: 344137]
Related plasmid reference: Sutcliffe, Cold Spring Harb. Symp. Quant. Biol. 43 (1979), 77-90 [PMID: 383387]
Peden, Gene 22 (1983), 277-280 [PMID: 6307828]
Lathe et al., DNA 3 (1984), 173-182 [PMID: 6327214]
Nugent et al., J. Gen. Microbiol. 132 (1986), 1021-1026 [PMID: 2428924]
Watson, Gene 70 (1988), 399-403 [PMID: 3063608]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 JMB9
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: DSM 3879
NCCB 3019
PC-V 3019

Refer in your Materials and Methods:

pBR322 (LMBP 140) is available at BCCM/GeneCorner. This plasmid was deposited by Dr F. Bolivar and was published in Bolivar et al., 1977.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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