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GeneCorner plasmid details

Last data update: 24 October 2020 04:12 CEST

Plasmid name: pBR322-mcrA (LMBP 10229)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Streptomyces lavendulae mitomycin C resistance cDNA (mcrA)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322
Further information: The plasmid was constructed by cloning the synthetic Streptomyces lavendulae mcrA coding sequence into the NotI/EcoRV opened pBR322 vector.
The mitomycin resistance gene can be used as a resistance marker in mammalian cells (Belcourt et al., 1999).
Other name of the plasmid is pBR322-NotI-mcrA-EcoRV.
EMBL Accession number: -
Latest sequence update: 12/04/2017
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Dr J. Staal(1)(2) and Prof. Dr R. Beyaert(1)(2).
(1) VIB Center for Inflammation Research, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Belcourt et al., Proc. Natl. Acad. Sci. U.S.A. 96 (1999), 10489-10494 [PMID: 10468636]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBR322-mcrA (LMBP 10229) is available at BCCM/GeneCorner. The plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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