Last data update: 24 January 2024 16:39 CET
Plasmid name: pBR322MLT (LMBP 2524)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2524.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse lymphotoxin A genomic DNA (Lta, LT) Escherichia coli modulation repressor |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322 |
| Further information: | The plasmid was constructed by inserting the genomic mouse lymphotoxin DNA (mLT) anticlockwise as a BamHI fragment into the unique BamHI site of pBR322. The mLT signal sequence can be isolated as a PvuII-ApaI fragment. |
| EMBL Accession number: | M16819, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 27/05/1991 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Ruddle(1). (1) Epidemiology & Public Health and Immunobiology, Yale University School of Medicine, New Haven, USA |
| Plasmid reference: | Li et al., J. Immunol. 138 (1987), 4496-4501 [PMID: 2884262] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBR322MLT (LMBP 2524) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Ruddle and was published in Li et al., 1987. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.