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GeneCorner plasmid details

Last data update: 25 October 2020 04:11 CET

Plasmid name: pBR322MLT (LMBP 2524)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2524.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse lymphotoxin A genomic DNA (Lta, LT)
Escherichia coli modulation repressor
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322
Further information: The plasmid was constructed by inserting the genomic mouse lymphotoxin DNA (mLT) anticlockwise as a BamHI fragment into the unique BamHI site of pBR322.
The mLT signal sequence can be isolated as a PvuII-ApaI fragment.
EMBL Accession number: M16819, view at EMBL, GenBank, DDBJ
Latest sequence update: 27/05/1991
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr N. Ruddle(1).
(1) Epidemiology & Public Health and Immunobiology, Yale University School of Medicine, New Haven, USA
Plasmid reference: Li et al., J. Immunol. 138 (1987), 4496-4501 [PMID: 2884262]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBR322MLT (LMBP 2524) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Ruddle and was published in Li et al., 1987.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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