Last data update: 23 November 2020 04:19 CET
Plasmid name: pBR322cI857 (LMBP 693)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Phage λ cI temperature-sensitive repressor gene (cIts, cI857)
|Promoter:||Phage λ major rightward promoter (λ PR)
Phage λ promoter for repressor maintenance (λ PRM)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pBR322; pcI857|
|Further information:||The plasmid was constructed by cloning the clts857 repressor gene as a HhaI fragment into the BamHI site of pBR322 via G/C-tailing. The cIts857 fragment is excisable as a BamHI fragment.
The length of the dG/dC tails is not known.
Other name of the plasmid is pVF1.
|EMBL Accession number:||-|
|Latest sequence update:||25/03/2011|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||PhD thesis Veronique Feys (1982)
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH1(λ)|
|Host reference:||Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBR322cI857 (LMBP 693) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Veronique Feys, 1982.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.