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GeneCorner plasmid details

Last data update: 23 November 2020 04:19 CET

Plasmid name: pBR322cI857 (LMBP 693)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p693.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage λ cI temperature-sensitive repressor gene (cIts, cI857)
Promoter: Phage λ major rightward promoter (λ PR)
Phage λ promoter for repressor maintenance (λ PRM)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322; pcI857
Further information: The plasmid was constructed by cloning the clts857 repressor gene as a HhaI fragment into the BamHI site of pBR322 via G/C-tailing. The cIts857 fragment is excisable as a BamHI fragment.
The length of the dG/dC tails is not known.
Other name of the plasmid is pVF1.
EMBL Accession number: -
Latest sequence update: 25/03/2011
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Veronique Feys (1982)
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH1(λ)
Host reference: Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBR322cI857 (LMBP 693) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Veronique Feys, 1982.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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