Last data update: 24 January 2024 16:39 CET
Plasmid name: pBR322cI857 (LMBP 693)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p693.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage λ cI temperature-sensitive repressor gene (cIts, cI857) |
| Promoter: | Phage λ major rightward promoter (λ PR) Phage λ promoter for repressor maintenance (λ PRM) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322; pcI857 |
| Further information: | The plasmid was constructed by cloning the clts857 repressor gene as a HhaI fragment into the BamHI site of pBR322 via G/C-tailing. The cIts857 fragment is excisable as a BamHI fragment. The length of the dG/dC tails is not known. Other name of the plasmid is pVF1. |
| EMBL Accession number: | - |
| Latest sequence update: | 25/03/2011 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Veronique Feys (1982) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH1(λ) |
| Host reference: | Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219 |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBR322cI857 (LMBP 693) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Veronique Feys, 1982. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.