Last data update: 24 January 2024 16:39 CET
Plasmid name: pBR325 (LMBP 324)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p324.gb
(View with Genome Compiler) p324.txt p324.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Tetracycline (tet) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322 |
| Further information: | This plasmid was derived from pBR322 by insertion of a 1.2 kb HaeII fragment (blunted with S1 nuclease) from phage P1Cm, containing the chloramphenicol resistance gene, into the unique EcoRI site (blunted with S1 nuclease). The plasmid carries, from position 739 to 1220, a 482 bp sequence which duplicates, in the opposite orientation, a section located at the end of the tetracycline gene (position 2045 to 2526). The nucleotide sequence of pBR325 corresponds with the EMBL Nucleotide Sequence Database accession number L08855.1, and adapted to the sequence of pBR322: two nucleotides between the tetracycline resistance gene and the origin were eliminated. |
| EMBL Accession number: | L08855.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/07/1990 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI and HindII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr F. Bolivar(1). (1) Universidad Nacional Autonoma de Mexico, Institute of Biotechnology, Cuernavaca, Morelos, Mexico |
| Plasmid reference: | Bolivar, Gene 4 (1978), 121-136 [PMID: 363519] |
| Related plasmid reference: | Prentki et al., Gene 14 (1981), 289-299 [PMID: 6271628] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 GM31 |
| Host reference: | Marinus, Mol. Gen. Genet. 127 (1973), 47-55 [PMID: 4589344] [DOI: 10.1007/BF00267782] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (25 μg/ml) + tetracycline (10 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin and tetracycline. |
| Other culture collection numbers: | DSM 3865 NCIB 11866 NCCB 3281 PC-V 3281 |
Refer in your Materials and Methods: |
pBR325 (LMBP 324) is available at BCCM/GeneCorner. This plasmid was deposited by Dr F. Bolivar and was published in Bolivar, 1978. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.