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GeneCorner plasmid details

Last data update: 25 October 2020 04:11 CET

Plasmid name: pBR325 (LMBP 324)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p324.gb (View with Genome Compiler)
p324.txt
p324.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322
Further information: This plasmid was derived from pBR322 by insertion of a 1.2 kb HaeII fragment (blunted with S1 nuclease) from phage P1Cm, containing the chloramphenicol resistance gene, into the unique EcoRI site (blunted with S1 nuclease).
The plasmid carries, from position 739 to 1220, a 482 bp sequence which duplicates, in the opposite orientation, a section located at the end of the tetracycline gene (position 2045 to 2526).
The nucleotide sequence of pBR325 corresponds with the EMBL Nucleotide Sequence Database accession number L08855.1, and adapted to the sequence of pBR322: two nucleotides between the tetracycline resistance gene and the origin were eliminated.
EMBL Accession number: L08855.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 30/07/1990
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI and HindII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr F. Bolivar(1).
(1) Universidad Nacional Autonoma de Mexico, Institute of Biotechnology, Cuernavaca, Morelos, Mexico
Plasmid reference: Bolivar, Gene 4 (1978), 121-136 [PMID: 363519]
Related plasmid reference: Prentki et al., Gene 14 (1981), 289-299 [PMID: 6271628]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 GM31
Host reference: Marinus et al., Mol. Gen. Genet. 127 (1973), 47-55 [PMID: 4589344]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (25 μg/ml) + tetracycline (10 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin and tetracycline.
Other culture collection numbers: DSM 3865
NCIB 11866
NCCB 3281
PC-V 3281

Refer in your Materials and Methods:

pBR325 (LMBP 324) is available at BCCM/GeneCorner. This plasmid was deposited by Dr F. Bolivar and was published in Bolivar, 1978.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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