Last data update: 27 November 2020 04:15 CET
Plasmid name: pBR325OMMBf (LMBP 2146)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human immunoglobulin γ3 cDNA (hIG3); fragment of the heavy chain encoding part of the human γ heavy chain disease (HCD) OMMB protein
|Selection marker:||Tetracycline (tet)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pBR325; pOMMB|
|Further information:||The plasmid was constructed by cloning the OMMB gene as a PstI fragment (nucleotide position 3645 and 4660) from pOMMB into the unique I-PstI site of pBR325.
As compared to the complete OMMB gene, the leader signal fragment and 3 codons of the variable region fragment are removed in the OMMBf gene.
The length of the dG/dC-tail after the 3' untranslated region is unknown.
The nucleotide sequence encoding the human γ heavy chain disease (HCD) OMM protein was obtained from Genbank 'Release 55; Locus : HUMIGHAF'. The unknown nucleotides in the Genbank-obtained sequence have been sequenced (Dr R. Schoonjans and K. De Sutter, Department of Biomedical Molecular Biology, Ghent University, Belgium). OMMBf differs from the total OMMB gene in that the Lf (leader signal fragment) and 3 amino acids of VHf are omitted, due to the PstI site in VHf (nucleotide position 3645 in pOMMB), used in the cloning procedure.
Other name of the plasmid is pBR325OMMB.
|EMBL Accession number:||J00231, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||21/01/1997|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + chloramphenicol (25 μg/ml) + tetracycline (10 μg/ml)*|
|Cultivation remark:||*: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with tetracycline.|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBR325OMMBf (LMBP 2146) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter .|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.