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GeneCorner plasmid details

Last data update: 27 November 2020 04:15 CET

Plasmid name: pBR325OMMBf (LMBP 2146)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2146.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human immunoglobulin γ3 cDNA (hIG3); fragment of the heavy chain encoding part of the human γ heavy chain disease (HCD) OMMB protein
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR325; pOMMB
Further information: The plasmid was constructed by cloning the OMMB gene as a PstI fragment (nucleotide position 3645 and 4660) from pOMMB into the unique I-PstI site of pBR325.
As compared to the complete OMMB gene, the leader signal fragment and 3 codons of the variable region fragment are removed in the OMMBf gene.
The length of the dG/dC-tail after the 3' untranslated region is unknown.
The nucleotide sequence encoding the human γ heavy chain disease (HCD) OMM protein was obtained from Genbank 'Release 55; Locus : HUMIGHAF'. The unknown nucleotides in the Genbank-obtained sequence have been sequenced (Dr R. Schoonjans and K. De Sutter, Department of Biomedical Molecular Biology, Ghent University, Belgium). OMMBf differs from the total OMMB gene in that the Lf (leader signal fragment) and 3 amino acids of VHf are omitted, due to the PstI site in VHf (nucleotide position 3645 in pOMMB), used in the cloning procedure.
Other name of the plasmid is pBR325OMMB.
EMBL Accession number: J00231, view at EMBL, GenBank, DDBJ
Latest sequence update: 21/01/1997
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + chloramphenicol (25 μg/ml) + tetracycline (10 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with tetracycline.
Other culture collection numbers: -

Refer in your Materials and Methods:

pBR325OMMBf (LMBP 2146) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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