Last data update: 24 January 2024 16:39 CET
Plasmid name: pBR325OMMBfmA (LMBP 2147)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2147.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human immunoglobulin γ3 cDNA (hIG3); fragment of the heavy chain encoding a mutated part of the human γ heavy chain disease (HCD) OMMB protein |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR325OMMBf |
| Further information: | This plasmid was derived from pBR325OMMBf via site-specific mutagenesis according to Morinaga et al., creating an ApaI site and a termination codon behind the fourth hinge region H4. Hence, the CH2 domain and the CH3 domain are no longer part of the coding information. The length of the dG/dC-tail after the 3' untranslated region is unknown. The nucleotide sequence encoding the human γ heavy chain disease (HCD) OMM protein was obtained from Genbank 'Release 55; Locus : HUMIGHAF'. The unknown nucleotides in the Genbank-obtained sequence have been sequenced (Dr R. Schoonjans and K. De Sutter, Department of Biomedical Molecular Biology, Ghent University, Belgium). Other name of the plasmid is pBR325OMMBA. |
| EMBL Accession number: | J00231, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 22/01/1997 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr K. De Sutter(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Morinaga et al., Biotechnology 2 (1984), 636-639 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + chloramphenicol (25 μg/ml) + tetracycline (10 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with tetracycline. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBR325OMMBfmA (LMBP 2147) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.