Last data update: 26 October 2020 04:25 CET
Plasmid name: pBR325OMMBfmA (LMBP 2147)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human immunoglobulin γ3 cDNA (hIG3); fragment of the heavy chain encoding a mutated part of the human γ heavy chain disease (HCD) OMMB protein
|Selection marker:||Tetracycline (tet)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||This plasmid was derived from pBR325OMMBf via site-specific mutagenesis according to Morinaga et al., creating an ApaI site and a termination codon behind the fourth hinge region H4. Hence, the CH2 domain and the CH3 domain are no longer part of the coding information.
The length of the dG/dC-tail after the 3' untranslated region is unknown.
The nucleotide sequence encoding the human γ heavy chain disease (HCD) OMM protein was obtained from Genbank 'Release 55; Locus : HUMIGHAF'. The unknown nucleotides in the Genbank-obtained sequence have been sequenced (Dr R. Schoonjans and K. De Sutter, Department of Biomedical Molecular Biology, Ghent University, Belgium).
Other name of the plasmid is pBR325OMMBA.
|EMBL Accession number:||J00231, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||22/01/1997|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Related plasmid reference:||Morinaga et al., Biotechnology 2 (1984), 636-639
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + chloramphenicol (25 μg/ml) + tetracycline (10 μg/ml)*|
|Cultivation remark:||*: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with tetracycline.|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBR325OMMBfmA (LMBP 2147) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter .|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.