Last data update: 24 January 2024 16:39 CET
Plasmid name: pBR327 (LMBP 1453)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1453.gb
(View with Genome Compiler) p1453.txt p1453.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322 |
| Further information: | The construction of the plasmid is described in Soberon et al. (1980). This plasmid is a pBR322 derivative in which the bom site as well as the region coding for the rop protein have been deleted. The plasmid cannot be mobilized. pBR327 displays a 2 to 3 fold higher copy number than pBR322. The nucleotide sequence of this plasmid is based on the EMBL Nucleotide Sequence Database accession number V00083.1, taking into account the revisions of pBR322. |
| EMBL Accession number: | V00083.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 01/12/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI and HindII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr F. Bolivar(1). (1) Universidad Nacional Autonoma de Mexico, Institute of Biotechnology, Cuernavaca, Morelos, Mexico |
| Plasmid reference: | Soberon et al., Gene 9 (1980), 287-305 [PMID: 6248430] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | DSM 5588 NCCB 3020 PC-V 3020 |
Refer in your Materials and Methods: |
pBR327 (LMBP 1453) is available at BCCM/GeneCorner. This plasmid was deposited by Dr F. Bolivar and was published in Soberon et al., 1980. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.