GeneCorner plasmid details

Last data update: 28 July 2021 09:51 CEST

Plasmid name: pBR327 (LMBP 1453)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: (View with Genome Compiler)
analysis results


Cloned DNA: -
Promoter: -
binding site:
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322
Further information: The construction of the plasmid is described in Soberon et al. (1980).
This plasmid is a pBR322 derivative in which the bom site as well as the region coding for the rop protein have been deleted. The plasmid cannot be mobilized.
pBR327 displays a 2 to 3 fold higher copy number than pBR322.
The nucleotide sequence of this plasmid is based on the EMBL Nucleotide Sequence Database accession number V00083.1, taking into account the revisions of pBR322.
EMBL Accession number: V00083.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 01/12/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI and HindII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr F. Bolivar(1).
(1) Universidad Nacional Autonoma de Mexico, Institute of Biotechnology, Cuernavaca, Morelos, Mexico
Plasmid reference: Soberon et al., Gene 9 (1980), 287-305 [PMID: 6248430]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: DSM 5588
NCCB 3020
PC-V 3020

Refer in your Materials and Methods:

pBR327 (LMBP 1453) is available at BCCM/GeneCorner. This plasmid was deposited by Dr F. Bolivar and was published in Soberon et al., 1980.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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