Last data update: 30 July 2021 10:22 CEST
Plasmid name: pBR328 (LMBP 1700)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pBR327; pBR325|
|Further information:||The plasmid was constructed by ligating the BamHI-PstI fragment of pBR325, containing the chloramphenicol resistance gene and part of the tetracycline and ampicillin resistance gene, to the BamHI-PstI fragment of pBR327, containing the origin of replication and the completing parts of the ampicillin and tetracycline resistance genes.
The plasmid carries, from position 4431 to 4907, a 477 bp sequence which duplicates, in the opposite orientation, a section located at the end of the tetracycline resistance gene (position 835 to 1311).
This plasmid is a pBR322 derivative in which the bom site as well as the region coding for the rop protein have been deleted. The plasmid cannot be mobilized.
The nucleotide sequence of pBR328 corresponds with the EMBL Nucleotide Sequence Database accession number L08858.1.
|EMBL Accession number:||L08858.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||29/08/1995|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: EcoRI and HindII.|
|History of deposit:||This plasmid was deposited by Dr F. Bolivar(1).
(1) Universidad Nacional Autonoma de Mexico, Institute of Biotechnology, Cuernavaca, Morelos, Mexico
|Plasmid reference:||Soberon et al., Gene 9 (1980), 287-305 [PMID: 6248430]
|Related plasmid reference:||Prentki et al., Gene 14 (1981), 289-299 [PMID: 6271628]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (25 μg/ml) + tetracycline (10 μg/ml)*|
|Cultivation remark:||*: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin and tetracycline.|
|Other culture collection numbers:||DSM 5589
Refer in your Materials and Methods:
|pBR328 (LMBP 1700) is available at BCCM/GeneCorner. This plasmid was deposited by Dr F. Bolivar and was published in Soberon et al., 1980.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.