Last data update: 22 January 2021 04:11 CET
Plasmid name: pBR329 (LMBP 768)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pBR327; pBR328|
|Further information:||This plasmid was derived from pBR327 by inserting a Hhal fragment op pBR328, containing the chloramphenicol resistance gene, into the EcoRI site. The cam gene lacks its own promoter but is transcribed from a promoter located in the vicinity of the HindIII site.
This plasmid is a pBR322 derivative in which the bom site as well as the region coding for the rop protein have been deleted. The plasmid can not be mobilized.
The nucleotide sequence of pBR329 corresponds with the EMBL Nucleotide Sequence Database accession number J01753.1; one nucleotide was added to correct the tet coding sequence and the pMB1 ori was adapted according the revisions of pBR322 (Watson, 1988).
|EMBL Accession number:||J01753.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||28/11/1991|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: EcoRI and HindII.|
|History of deposit:||This plasmid was deposited by Dr F. Bolivar(1).
(1) Universidad Nacional Autonoma de Mexico, Institute of Biotechnology, Cuernavaca, Morelos, Mexico
|Plasmid reference:||Covarrubias et al., Gene 17 (1982), 79-89 [PMID: 6281139]
|Related plasmid reference:||Watson, Gene 70 (1988), 399-403 [PMID: 3063608]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 GM31|
|Host reference:||Marinus et al., Mol. Gen. Genet. 127 (1973), 47-55 [PMID: 4589344]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (25 μg/ml) + tetracycline (10 μg/ml)*|
|Cultivation remark:||*: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin and tetracycline.|
|Other culture collection numbers:||DSM 5590
Refer in your Materials and Methods:
|pBR329 (LMBP 768) is available at BCCM/GeneCorner. This plasmid was deposited by Dr F. Bolivar and was published in Covarrubias et al., 1982.|