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GeneCorner plasmid details

Last data update: 22 October 2020 04:25 CEST

Plasmid name: pBRE6H (LMBP 1678)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pbre6h.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; heavy chain (E6H)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322
Further information: E6H is a cDNA copy of the heavy chain of the mouse monoclonal antibody E6(IgG2b,κ) which was cloned in the unique PstI site of pBR322 by dG/dC-tailing.
The 5' untranslated region of E6H consists of 25 dG-residues (tail) and 51 nucleotides from 5UTR.
The 3' untranslated region of E6H consists of 114 nucleotides from 3UTR and 14 dC-residues (tail).
A second isolated clone named pBRE6H2 (storage number: 1679) differs from pBRE6H only in the length of the 5' and 3' untranslated regions.
Name of he plasmid mentioned in Feys et al. (1988) is pBRE6-H.
EMBL Accession number: -
Latest sequence update: 22/03/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by P. De Waele(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: De Waele et al., Eur. J. Biochem. 176 (1988), 287-295 [PMID: 3138116]
Related plasmid reference: Feys et al., Int. J. Cancer Suppl. 2 (1988), 26-27 [PMID: 3162443]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRE6H (LMBP 1678) is available at BCCM/GeneCorner. This plasmid was deposited by P. De Waele and Prof. Dr W. Fiers and was published in De Waele et al., 1988.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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