Last data update: 22 October 2020 04:25 CEST
Plasmid name: pBRE6H (LMBP 1678)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; heavy chain (E6H)
|Selection marker:||Tetracycline (tet)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||E6H is a cDNA copy of the heavy chain of the mouse monoclonal antibody E6(IgG2b,κ) which was cloned in the unique PstI site of pBR322 by dG/dC-tailing.
The 5' untranslated region of E6H consists of 25 dG-residues (tail) and 51 nucleotides from 5UTR.
The 3' untranslated region of E6H consists of 114 nucleotides from 3UTR and 14 dC-residues (tail).
A second isolated clone named pBRE6H2 (storage number: 1679) differs from pBRE6H only in the length of the 5' and 3' untranslated regions.
Name of he plasmid mentioned in Feys et al. (1988) is pBRE6-H.
|EMBL Accession number:||-|
|Latest sequence update:||22/03/1989|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by P. De Waele(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||De Waele et al., Eur. J. Biochem. 176 (1988), 287-295 [PMID: 3138116]
|Related plasmid reference:||Feys et al., Int. J. Cancer Suppl. 2 (1988), 26-27 [PMID: 3162443]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRE6H (LMBP 1678) is available at BCCM/GeneCorner. This plasmid was deposited by P. De Waele and Prof. Dr W. Fiers and was published in De Waele et al., 1988.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.