Last data update: 24 January 2024 16:39 CET
Plasmid name: pBRE6H2 (LMBP 1679)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1679.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; heavy chain (E6H) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322 |
| Further information: | Sequencing the E6H2 gene was a confirmation for the DNA sequence of the E6H gene. E6H2 is a second characterised cDNA copy of the heavy chain of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) which was cloned in the unique PstI site of pBR322 by dG/dC-tailing. The main differences with pBRE6H are : 1) the clockwise orientation of the E6H2 gene in pBRE6H2; 2) the presence of an AvaII site (GGACC) in the 5' untranslated region (5UTR) as a result of [(dG)24]ACCT; 3) the absence of a KpnI site in the 3' untranslated region. Other name of the plasmid is pBRE6H12. |
| EMBL Accession number: | - |
| Latest sequence update: | 22/03/1989 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by P. De Waele(1) and Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | De Waele et al., Eur. J. Biochem. 176 (1988), 287-295 [PMID: 3138116] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBRE6H2 (LMBP 1679) is available at BCCM/GeneCorner. This plasmid was deposited by P. De Waele and Prof. Dr W. Fiers and was published in De Waele et al., 1988. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.