Last data update: 22 October 2020 04:25 CEST
Plasmid name: pBRE6H2 (LMBP 1679)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; heavy chain (E6H)
|Selection marker:||Tetracycline (tet)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||Sequencing the E6H2 gene was a confirmation for the DNA sequence of the E6H gene.
E6H2 is a second characterised cDNA copy of the heavy chain of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) which was cloned in the unique PstI site of pBR322 by dG/dC-tailing.
The main differences with pBRE6H are :
1) the clockwise orientation of the E6H2 gene in pBRE6H2;
2) the presence of an AvaII site (GGACC) in the 5' untranslated region (5UTR) as a result of [(dG)24]ACCT;
3) the absence of a KpnI site in the 3' untranslated region.
Other name of the plasmid is pBRE6H12.
|EMBL Accession number:||-|
|Latest sequence update:||22/03/1989|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by P. De Waele(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||De Waele et al., Eur. J. Biochem. 176 (1988), 287-295 [PMID: 3138116]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRE6H2 (LMBP 1679) is available at BCCM/GeneCorner. This plasmid was deposited by P. De Waele and Prof. Dr W. Fiers and was published in De Waele et al., 1988.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.