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GeneCorner plasmid details

Last data update: 25 October 2020 04:11 CET

Plasmid name: pBRE6L2 (LMBP 1681)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1681.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; light chain (E6L)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322
Further information: Sequencing the E6L2 gene was a confirmation for the DNA sequence of the E6L gene.
E6L2 is a second characterised cDNA copy of the light chain of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) which was cloned in the unique PstI site of pBR322 by dG/dC-tailing.
The main differences with pBRE6L are :
1) the clockwise orientation of the E6L2 gene in pBRE6L2;
2) the 3' untranslated region of E6L2 is 22 nucleotides longer;
3) the 5' untranslated region of E6L2 is 17 nucleotides shorter.
Other name of the plasmid is pBRE6LE1.
EMBL Accession number: -
Latest sequence update: 21/03/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: De Waele et al., Eur. J. Biochem. 176 (1988), 287-295 [PMID: 3138116]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRE6L2 (LMBP 1681) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers and was published in De Waele et al., 1988.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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