Last data update: 24 January 2024 16:39 CET
Plasmid name: pBRHIFNBg4 (LMBP 1622)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1622.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interferon β gene (IFNB, IFNB1) |
| Promoter: | Human interferon β promoter (IFNB) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR325 |
| Further information: | This plasmid contains the genomic EcoRI fragment of the human interferon β (hIFNB) gene with its 3' and 5' flanks, from lambdaCH4AgHIFNB1, inserted into the EcoRI site of pBR325. The hIFNB gene contains no introns. Name mentioned in Tavernier et al. (1981) is p(325)-gHFIF-4. Other names of the plasmid are pg4, p(325)gHFIF4 and pBR325gHIFNB4. |
| EMBL Accession number: | - |
| Latest sequence update: | 26/03/1992 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by J. Tavernier(1) and Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Tavernier et al., Nucleic Acids Res. 9 (1981), 461-471 [PMID: 6164047] |
| Related plasmid reference: | Derynck et al., Nature 285 (1980), 542-547 [PMID: 6157094] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBRHIFNBg4 (LMBP 1622) is available at BCCM/GeneCorner. This plasmid was deposited by J. Tavernier and Prof. Dr E. Remaut and was published in Tavernier et al., 1981. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.