GeneCorner plasmid details

Last data update: 20 September 2021 09:16 CEST

Plasmid name: pBRHIFNBg4 (LMBP 1622)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence:
analysis results


Cloned DNA: Human interferon β gene (IFNB, IFNB1)
Promoter: Human interferon β promoter (IFNB)
binding site:
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR325
Further information: This plasmid contains the genomic EcoRI fragment of the human interferon β (hIFNB) gene with its 3' and 5' flanks, from lambdaCH4AgHIFNB1, inserted into the EcoRI site of pBR325.
The hIFNB gene contains no introns.
Name mentioned in Tavernier et al. (1981) is p(325)-gHFIF-4.
Other names of the plasmid are pg4, p(325)gHFIF4 and pBR325gHIFNB4.
EMBL Accession number: -
Latest sequence update: 26/03/1992
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by J. Tavernier(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Tavernier et al., Nucleic Acids Res. 9 (1981), 461-471 [PMID: 6164047]
Related plasmid reference: Derynck et al., Nature 285 (1980), 542-547 [PMID: 6157094]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRHIFNBg4 (LMBP 1622) is available at BCCM/GeneCorner. This plasmid was deposited by J. Tavernier and Prof. Dr E. Remaut and was published in Tavernier et al., 1981.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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