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GeneCorner plasmid details

Last data update: 24 October 2020 04:12 CEST

Plasmid name: pBRHIFNBg41 (LMBP 1659)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1659.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interferon β gene (IFNB, IFNB1)
Promoter: Human interferon β promoter (IFNB)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBRHIFNBg4
Further information: Contains the genomic hIFNB gene with 3' and 5' flanks.
HIFNB GENE CONTAINS NO INTRONS.
This plasmid is derived from pBRHIFNBg4 by deleting a HindIII fragment containing non-essential vector DNA and part of the 3' flanking region of the hIFNβ gene. This results in an unique EcoRI site before the promoter and the gene.
The tetracycline gene is presumably not biologically active.
Other names of the plasmid are pg4delH and pBR325gHIFNB41.
EMBL Accession number: -
Latest sequence update: 26/03/1992
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by J. Tavernier(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Tavernier et al., Nucleic Acids Res. 9 (1981), 461-471 [PMID: 6164047]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRHIFNBg41 (LMBP 1659) is available at BCCM/GeneCorner. This plasmid was deposited by J. Tavernier and Prof. Dr E. Remaut.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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