Last data update: 24 January 2024 16:39 CET
Plasmid name: pBRHIFNBg41 (LMBP 1659)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1659.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interferon β gene (IFNB, IFNB1) |
| Promoter: | Human interferon β promoter (IFNB) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBRHIFNBg4 |
| Further information: | Contains the genomic hIFNB gene with 3' and 5' flanks. HIFNB GENE CONTAINS NO INTRONS. This plasmid is derived from pBRHIFNBg4 by deleting a HindIII fragment containing non-essential vector DNA and part of the 3' flanking region of the hIFNβ gene. This results in an unique EcoRI site before the promoter and the gene. The tetracycline gene is presumably not biologically active. Other names of the plasmid are pg4delH and pBR325gHIFNB41. |
| EMBL Accession number: | - |
| Latest sequence update: | 26/03/1992 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by J. Tavernier(1) and Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Tavernier et al., Nucleic Acids Res. 9 (1981), 461-471 [PMID: 6164047] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBRHIFNBg41 (LMBP 1659) is available at BCCM/GeneCorner. This plasmid was deposited by J. Tavernier and Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.