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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pBRHIFNGf0 (LMBP 1274)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p1274.gb (View with Genome Compiler)
p1274.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interferon γ cDNA (IFNG); fragment
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR327
Further information: This plasmid was constructed by inserting the dG/dC-tailed fragment of the human interferon γ cDNA (γ-0) into the unique (filled-in) BamHI site of pBR327. The coding sequence of the hIFNG cDNA is not complete: the fragment starts (presumably) at codon 17 of the mature sequence. The orientation and the exact size of the inserted fragment are not known.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727573.1.
Other name of the plasmid is pBR327γ0-2.
EMBL Accession number: LT727573.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 02/12/1993
Sequence detail:
Presumable start of the sequence of the gamma-0 clone:

               17  18  19  20  21
... CCCCCCCCC GCA GGT CAT TCA GAT G ...
              Ala Gly His Ser Asp
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, BglI and EcoRI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr R. Devos(1).
(1) Roche Research Gent, Belgium
Plasmid reference: -
Related plasmid reference: Devos et al., Nucleic Acids Res. 10 (1982), 2487-2501 [PMID: 6176945]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH1(λ)
Host reference: Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRHIFNGf0 (LMBP 1274) is available at BCCM/GeneCorner. This plasmid was deposited by Dr R. Devos.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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