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GeneCorner plasmid details

Last data update: 02 December 2020 04:29 CET

Plasmid name: pBRST10 (LMBP 1805)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pbrst10.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Satellite tobacco necrosis virus (STNV)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function
Parental clone: pPLST10; pBR322
Further information: The plasmid was constructed as follows: the EcoRI-BglI fragment of pBR322, containing the ampicillin resistance gene, was inserted into the larger EcoRI-BglI fragment of pPLST10, containing the STNV information and the Eco-pMB1 origin.
The STNV fragment is complete.
Name mentioned in Van Emmelo et al. (1987) is pBRSTNV403.
Other name of the plasmid is pSTBR1.
EMBL Accession number: -
Latest sequence update: 19/09/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Van Emmelo et al., Virology 157 (1987), 480-487 [PMID: 18644559]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 r-m+ (λ)
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRST10 (LMBP 1805) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Van Emmelo et al., 1987.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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