Last data update: 24 January 2024 16:39 CET
Plasmid name: pBRST10 (LMBP 1805)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | pbrst10.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Satellite tobacco necrosis virus (STNV) |
Promoter: | - |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli; use strains with a cI function |
Parental clone: | pPLST10; pBR322 |
Further information: | The plasmid was constructed as follows: the EcoRI-BglI fragment of pBR322, containing the ampicillin resistance gene, was inserted into the larger EcoRI-BglI fragment of pPLST10, containing the STNV information and the Eco-pMB1 origin. The STNV fragment is complete. Name mentioned in Van Emmelo et al. (1987) is pBRSTNV403. Other name of the plasmid is pSTBR1. |
EMBL Accession number: | - |
Latest sequence update: | 19/09/1989 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | Van Emmelo et al., Virology 157 (1987), 480-487 [PMID: 18644559] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 r-m+ (λ) |
Host reference: | - |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBRST10 (LMBP 1805) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Van Emmelo et al., 1987. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.