Last data update: 02 December 2020 04:29 CET
Plasmid name: pBRST10 (LMBP 1805)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Satellite tobacco necrosis virus (STNV)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function|
|Parental clone:||pPLST10; pBR322|
|Further information:||The plasmid was constructed as follows: the EcoRI-BglI fragment of pBR322, containing the ampicillin resistance gene, was inserted into the larger EcoRI-BglI fragment of pPLST10, containing the STNV information and the Eco-pMB1 origin.
The STNV fragment is complete.
Name mentioned in Van Emmelo et al. (1987) is pBRSTNV403.
Other name of the plasmid is pSTBR1.
|EMBL Accession number:||-|
|Latest sequence update:||19/09/1989|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Van Emmelo et al., Virology 157 (1987), 480-487 [PMID: 18644559]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 r-m+ (λ)|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRST10 (LMBP 1805) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Van Emmelo et al., 1987.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.